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rabbit polyclonal anti brca1  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit polyclonal anti brca1
    Rabbit Polyclonal Anti Brca1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+brca1+ser1387/BRCA1+%5Bp+Ser1387%5D+Antibody+%5BAllophycocyanin%5D/pm40402574-161-22-27
    Average 93 stars, based on 2 article reviews
    rabbit polyclonal anti brca1 - by Bioz Stars, 2026-09
    93/100 stars

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    Staining:

    Article Title: Acid Sphingomyelinase-Ceramide Induced Vascular Injury Determines Colorectal Cancer Stem Cell Fate
    Article Snippet: Thereafter, slides, incubated overnight with primary Abs at 4 °C to stain DNA repair foci (see below), were washed 5 × 5’ in PBS-Triton on a shaker, incubated in goat anti-mouse or anti-rabbit IgG (H+L) cross-adsorbed secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, A-11070 and A-11017; 2 mg/ml, dilution 1:400) for 1 h at room temperature, and washed with 1 × 5’ in PBS-Triton and 4 × 5’ in PBS on a shaker. .. Primary antibodies used to stain DNA repair proteins are as follows: mouse monoclonal anti-γH2AX-phosphoSer139 (Milli-pore [clone JBW301] 05–636; dilution 1:1000), rabbit polyclonal anti-BRCA1 (Ser1387) (Novus Biologicals, NB100–225SS; dilution 1:250), and mouse monoclonal anti-DNA-PKcs (phospho-Thr2609) (Abcam, ab18356; dilution 1:250). .. Slides were mounted in ThermoFisher ProLong TM Diamond Antifade Mountant with DAPI and sealed with nail polish before imaging.

    Article Title: Acid Sphingomyelinase-Ceramide Induced Vascular Injury Determines Colorectal Cancer Stem Cell Fate.
    Article Snippet: Thereafter, slides, incubated overnight with primary Abs at 4 °C to stain DNA repair foci (see below), were washed 5 x 5’ in PBS-Triton on a shaker, incubated in goat antimouse or anti-rabbit IgG (H+L) cross-adsorbed secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific, A-11070 and A-11017; 2 mg/ml, dilution 1:400) for 1 h at room temperature, and washed with 1 x 5’ in PBS-Triton and 4 x 5’ in PBS on a shaker. .. Primary antibodies used to stain DNA repair proteins are as follows: mouse monoclonal anti-γH2AX-phosphoSer139 (Milli-pore [clone JBW301] 05-636; dilution 1:1000), rabbit polyclonal anti-BRCA1 (Ser1387) (Novus Biologicals, NB100-225SS; dilution 1:250), and mouse monoclonal anti-DNA-PKcs (phospho-Thr2609) (Abcam, ab18356; dilution 1:250). .. Slides were mounted in ThermoFisher ProLongTM Diamond Antifade Mountant with DAPI and sealed with nail polish before imaging.



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    Novus Biologicals rabbit polyclonal anti brca1
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